Journal: Nucleic Acids Research

Article Title: Nrf2–MafG heterodimers contribute globally to antioxidant and metabolic networks

doi: 10.1093/nar/gks827

Figure Lengend Snippet: DEM-inducible genes proximal to Nrf2–MafG and Nrf2 single binding sites. Microarray analysis was performed with RNA isolated from DEM- or DMSO-treated Hepa1 cells for 6 h in duplicate. ( A ) Venn diagram showing the overlap of genes induced by DEM (≥1.5-fold change) and genes proximal to Nrf2–MafG or Nrf2 single binding sites. ( B ) Venn diagram showing the overlap of genes repressed by DEM (≥1.5-fold change) and genes proximal to the Nrf2–MafG or Nrf2 single binding sites. ( C , D ) Heat map of differentially expressed genes proximal to the Nrf2–MafG-binding sites (C) or Nrf2 single binding sites (D). DEM-induced genes proximal to Nrf2–MafG-binding sites are categorized into functional groups: antioxidant and detoxification enzymes, proteasome and chaperone, transporter, metabolism and others. The colors of the heat map reflect the log (2) -fold-change values relative to the mean expression level of each gene in the DMSO-treated (Veh) Hepa1 cells. The gene symbols used here are consistent with those used in the Mouse Genome Informatics database.

Article Snippet: The Agilent 4 × 44 K Whole-Mouse Genome Oligo Microarray slides were hybridized, washed and scanned on an Agilent Microarray Scanner according to the Agilent protocol.

Techniques: Binding Assay, Microarray, Isolation, Functional Assay, Expressing

Journal: Oncogene

Article Title: EPLIN Downregulation Promotes Epithelial-Mesenchymal Transition in Prostate Cancer Cells and Correlates With Clinical Lymph Node Metastasis

doi: 10.1038/onc.2011.199

Figure Lengend Snippet: (A) Microarray analysis of gene expression profile in ARCaP E -pRS and ARCaP E -shRNA cells. (B) Selected genes affected by EPLIN depletion in ARCaP E cells. (C) Validation of several putative EPLIN target genes. Left panel: RT-PCR analysis of the effects of EPLIN depletion on the expression of several selected genes in ARCaP E cells. Right panel: Western blot analysis of the effects of EPLIN depletion on protein expression in the total lysates (top) and nuclear extracts (bottom) in ARCaP E cells. (D) Effects of EPLIN depletion on the expression of active MMP-27 in ARCaP E cells, as analyzed by gelatin zymogram. (E) Effects of EPLIN depletion on the membrane expression of CD44 in ARCaP E cells, as analyzed by fluorescence-activated Cell Sorting (FACS). Y -axis: cell numbers. (F) Real-time qPCR analysis of the effects of EPLIN depletion on the expression of miR-200b, miR-429 and miR-205 in ARCaP E cells.

Article Snippet: Total RNA from triplicate preparations of control and knockdown samples as well as reference total RNA samples were amplified and hybridized to Agilent 44K whole human genome expression oligonucleotide microarray slides as previously described ( ).

Techniques: Microarray, Expressing, shRNA, Reverse Transcription Polymerase Chain Reaction, Western Blot, Fluorescence, FACS

Journal: Oncogene

Article Title: EPLIN Downregulation Promotes Epithelial-Mesenchymal Transition in Prostate Cancer Cells and Correlates With Clinical Lymph Node Metastasis

doi: 10.1038/onc.2011.199

Figure Lengend Snippet: (A) Microarray data-mining of EPLIN transcript expression in primary and metastatic PCa. (B) IHC expression of EPLIN were examined in matched pairs of specimens from primary and lymph node metastatic PCa and compared for the statistical significance. Bars denote the standard error.

Article Snippet: Total RNA from triplicate preparations of control and knockdown samples as well as reference total RNA samples were amplified and hybridized to Agilent 44K whole human genome expression oligonucleotide microarray slides as previously described ( ).

Techniques: Microarray, Expressing

Journal: Oncogene

Article Title: EPLIN Downregulation Promotes Epithelial-Mesenchymal Transition in Prostate Cancer Cells and Correlates With Clinical Lymph Node Metastasis

doi: 10.1038/onc.2011.199

Figure Lengend Snippet: (A) Microarray data-mining of EPLIN transcript expression in primary and metastatic colorectal cancer. IHC expression of EPLIN were examined in matched pairs of tumor tissues specimens or TMAs and compared for the statistical significance in human colorectal cancer (B), breast cancer (C) and SCCHN (D). Bars denote the standard error.

Article Snippet: Total RNA from triplicate preparations of control and knockdown samples as well as reference total RNA samples were amplified and hybridized to Agilent 44K whole human genome expression oligonucleotide microarray slides as previously described ( ).

Techniques: Microarray, Expressing